Results – Evaluation of the ocular irritation – GREENQUAT®BT
The obtained results are reported in tables and charts containing the measurements of cell viability, evaluated by MTT assay, on an in vitro 3D reconstructed human corneal epidermis (HCE, SkinEthicTM).
Evaluation of cell viability
The purpose of the test is to evaluate the possible eye irritation of the tested product after 30 minutes of exposure by assessing the viability of the tissues (HCE, SkinEthic).
Figure and Table show the graph and corresponding data of cell viability expressed as a percentage of positive control (methyl acetate) and the product compared to the negative control (CTRL-, cells treated with DPBS).
Figure: Effect of 30 min exposure with methyl acetate (positive control) and the tested product on cell viability (MTT test). (n=1; replicates=3).
| Sample | Cell viability (%) ± standard deviation |
| CTRL – | 100 ± 6.96 |
| CTRL + | 26.78 ± 2.12 |
| GREENQUAT BT 5% |
82.64 ± 4.15 |
Table: Percentage changes in cell viability after 30 min treatment with positive control and the product respect to negative control (CTRL-, cells treated with DPBS).
The obtained results demonstrate that GREENQUAT®BT 5%, at the tested concentration, does not show a cytotoxic effect after 30 minutes of contact on 3D in vitro reconstructed human corneal epidermis (HCE, SkinEthicTM), having a cell viability > 60%, threshold used to identify a substance as not dangerous or irritating for eye. The positive control, methyl acetate, shows a reduced cell viability compared to the control.